Intra-cytoplasmic sperm injection (ICSI) is one of the Best assisted reproductive technologies (ART) mainly used especially for male infertility causes such as oligospermia,teratozoospermia, asthenozoospermia, in azoospermia in which sperm was retrieved through Testicular sperm aspiration. ICSI treatment in Southend, differs from conventional invitro fertilisation (IVF). The process involves fertilization of the egg by  inserting a single sperm into a mature egg which is done in the embryology laboratory using highly sensitive micromanupulation inverted microscopes, the embryos resulting are then places in the incubator for 3days or 5 days before transferring into the  woman’s uterus for subsequent implantation.